Figure 6.

Binding of the anti-α2Ct scFv and the chIgG variants to the collagen fibrils. In the demonstrated fibrils arrows point to the gold particles representing positions of the fibril-bound scFv or chIgG constructs. Included inserts represent magnified views of the gap regions with gold particles representing fibril-bound antibody variants. Control (Ctrl) fibrils not exposed to the anti-α2Ct constructs show no binding of the gold particles.

Lower portion of this figure depicts a telopeptide-region with a possible binding site (asterisk) of the anti-α2Ct constructs presented in the context of the specific arrangement of collagen molecules that form a collagen microfibril. Specific regions corresponding to particular D-periods of the collagen molecule are also indicated.