Figure 7.

Effects of the presence of the anti-α2Ct scFv variant on the deposition of collagen fibrils and viability of cells isolated from tendon (T) and tendon sheath (Ts). A: An experimental setting of co-cultures of employed cells is indicated. B: In the presence of the scFv (+scFv), no collagen deposits have been formed in the region separating layers of cells derived from tendon and tendon sheath. In contrast, in the absence of the scFv variant (−scFv), collagen-rich deposits have been formed in the border region. Control (Ctrl) depicts a border region in a sample in which a dividing barrier has not been removed. Dotted lines indicate the borders of cell layers formed by T and Ts cells and define a region separating them. C: The viability of cells has been analyzed as the function of increasing concentrations of the scFv construct. D: The utility of the employed test was validated by the observation of viability reduction in response to the increasing concentrations of TNFα. E, F: The effects of the anti-α2Ct scFv on the proliferation of tendon-derived (E) and tendon sheath-derived (F) cells has been analyzed in comparison to untreated control (Ctrl) and to control treated with actinomycin D (Actin-D).