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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: J Thromb Haemost. 2006 Jun;4(6):1218–1225. doi: 10.1111/j.1538-7836.2006.01901.x

Fig. 5.

Fig. 5

Persistence of vector-specific DNA in liver after administration of HDV-PEPCK/BDD-cFVIII/WPRE. (A) Vector-specific Southern blot. Genomic DNA from days 18 and 79 liver biopsy tissue was probed with vector-specific WPRE element. Control is normal canine genomic DNA. (B) Semi-quantitative polymerase chain reaction (PCR) with WPRE and beta-actin-specific primers. BA is β actin control, C is DNA derived from a normal dog, W is WPRE primer run in water, and BA* is β actin-specific primers run in water. (C) RT-PCR and qualitative RT-PCR from days 18 and 79 liver biopsy tissue were performed with vector-specific WPRE primers. Naïve indicates an untreated FVIII-deficient animal, W is water control.