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. 2014 Feb 17;2014:926130. doi: 10.1155/2014/926130

Figure 5.

Figure 5

FC-98 inhibited activation of MAPK and STAT1 to downregulate the CXCL-10 expression. (a) Left: BMDCs were pretreated with FC-98 for 2 h, followed by 30 min CpG treatment; the phosphorylation of of MAPK (ERK, JNK, and p38) signaling pathway was analyzed by western blot. The results shown are representative experiments from three independent assays. Right: BMDCs were cotransfected with 100 ng pAP-1 luciferase reporter plasmid and 10 ng pRL-TK-Renilla luciferase. Total amounts of plasmid DNA were equalized using empty control vector. After 24 h of culture, cells were pretreated with FC-98 for 1 h and then stimulated with 1 μM CpG for another 6 h. Luciferase activity was measured and normalized by Renilla luciferase activity. Data are shown as mean ± SD of three independent assays. ### P < 0.001 versus control group; *P < 0.05, ***P < 0.001 versus CpG-only group. (b) Left: experiments were duplicated as described in (a), left, and phosphorylated STAT1 (p-STAT1) and total STAT1 were detected by western blot. The results shown are representative experiments from three independent assays. Right: the results of western blot were analysed by Image J, and the relative intensity was showed. ### P < 0.001 versus control group; ∗∗P < 0.01 versus 2 (CpG-only group).