Figure 1.

Purification and chemical modification of secretory phospholipase A₂ (sPLA₂). A fractionation of the whole venom was performed by reverse-phase HPLC (C5 column, 0.10 cm × 25 cm) using a nonlinear concentration gradient of buffer to obtain a high-purity protein. (a) shows a comparative profile of native sPLA₂, sPLA₂ : Q, and sPLA₂ : Qn when subjected to reverse-phase HPLC. (b) shows the MALDI-TOF mass spectrometry analysis of native sPLA₂ and sPLA₂ : Qn, indicating the difference in the molecular mass corresponding to one molecule of bound quercitrin.