Table 1.
Primer name | Primer sequence (5′–3′) | Annealing |
---|---|---|
Primer-1 | AAGAATTCGAAGGACGCAGCAGAAGC [24] | 30 s at 52 °C |
Primer-2 | CAGAATTCOTCATGTACGCTGGCAC [24] | |
Primer-3 | TCGGTCCCCAAAGCAAAGGTCA [24] | 30 s at 55 °C |
Primer-4 | CATTCACGGCATCCCAGTCAGC [24] | |
Primer-5 | TGGTGCGTGGAGCAAGGAAA | 30 s at 55 °C |
Primer-6 | TTCACCTTCAGCGCTGGGAA |