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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: Nat Cell Biol. 2011 Oct 3;13(10):1154–1160. doi: 10.1038/ncb2345

Figure 2.

Figure 2

Identification of SAS-6 as a key element of the centriolar cartwheel. (a) Immunolocalization of the SAS-6 protein (also known as Bld12p) to the central hub of the cartwheel in Chlamydomonas reinhardtii imaged by electron microscopy. Top images show longitudinal sections through wild-type centrioles; note the immunogold-labelling of the carthwheel by anti-SAS-6 antibodies (right). Bottom; immunogold-labelling of centriole in cross-section, showing that SAS-6 localizes to the central part of the cartwheel (right). Schematic representation (left) shows cartwheel in red. Scale bars,100 nm. Reproduced with permission from ref. 35. (b). Structural model of a SAS-6 oligomer (upper panel) and rotary-metalshadowing electron micrographs of the same structure (lower right panel; schematic representation of structure is shown on the left). Both images emphasize the importance of SAS-6 for conferring ninefold rotational symmetry to the centriole. (Reproduced with permission from ref. 38).