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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):380–392. doi: 10.1016/j.molcel.2007.05.041

Figure 4. Collaborative Transcription Factors Are Recruited to AR Binding Regions.

Figure 4

(A) Schematic representation of association of AR half-site and transcription factor motifs within AR binding regions. Each pairwise association is designated by a circle with different color. The area of the circle is proportional to the frequency of the association.

(B) The recruitment of collaborative factors to AR binding regions. LNCaP cells were cultured in hormone-depleted medium for 3 days and then treated with vehicle or DHT for 1 and 16 hr. ChIP analyses were then performed with indicated antibodies or control IgG. DNA precipitates were measured by real-time PCR using primers spanning the PSA enhancer and nine AR binding regions on chromosomes 21 and 22. The results are shown as the average of two to three replicates ± SE. The occurrence of ARE, Forkhead, GATA, and Oct motifs in each region is also shown.