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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: Mol Microbiol. 2012 Aug 22;86(2):284–302. doi: 10.1111/j.1365-2958.2012.08193.x

Figure 8. Cell wall depth visualization.

Figure 8

Transmission electron microscopy of sectioned cells was used to determine cell wall thickness on 10 unselected cells of each strain, with means and standard deviations indicated. Representative images are shown. (A) The wild-type (SC5314) cell wall measurement was 92nm+/−22nm. (B) The tpk1Δ/tpk1Δ (SF1358) cell wall measurement was 48nm+/−22nm. p=<0.0001 relative to wild type. (C) The tpk1Δ/tpk1Δ +TPK1 (SF1119) cell wall measurement was 74nm+/−31nm. p=0.04 relative to tpk1Δ/tpk1Δ and not statistically significant (p=0.08) from wild type. (D) The wild-type strain overexpressing ALS2 (SF1127) cell wall measurement was 39nm +/− 14nm. p=<0.0001 relative to wild-type. (E) A heterozygous ALS2 deletion strain (SF1332) had a cell wall measurement of 111nm +/− 29nm. p=0.04 relative to wild type. (F) The wild-type strain overexpressing ALS4 (SF1129) cell wall measurement was 75nm +/− 24nm. p= 0.06 relative to wild type.