Table 2.
Primers used in this study
| Primer Name | Primer Sequence | |
|---|---|---|
| TPK1-5DR | AGATCAATATATCCTATCGTTATCCTCCTCCTTCTCCTTTCAACTTTTGAAAAAGGTGATATTATTCAACACTGTTTCTGTTTTATCAACCAAAACCAGGTTTCCCAGTCACGACGTT | This study |
| TPK1-3DR | TCTTTTTTCTTGTTCTTTCTTGCAAATTAATTATACATCTATAAAACTAGTTATCATAATTAACATTGTTGTGCCAATAAATACAATTTTATTTTTACATGTGGAATTGTGAGCGGATA | This study |
| TDH3F | ATCCCACAAGGACTGGAGA | Blankenship et al., 2010 |
| TDH3R | GCAGAAGCTTTAGCAACGTG | Blankenship et al., 2010 |
| ALS1F | GACTAGTGAACCAACAAATACCAGA | Green et al., 2004 |
| ALS1R | CCAGAAGAAACAGCAGGTGA | Green et al., 2004 |
| ALS2F | CCAAGTATTAACAAAGTTTCAATCACTTAT | Green et al., 2004 |
| ALS2R | TCTCAATCTTAAATTGAACGGCTTAC | Green et al., 2004 |
| ALS3F | CCACTTCACAATCCCCATC | Green et al., 2004 |
| ALS3R | CAGCAGTAGTAGTAACAGTAGTAGTTTCATC | Green et al., 2004 |
| ALS4F | CCCAGTCTTTCACAAGCAGTAAAT | Green et al., 2004 |
| ALS4R | GTAAATGAGTCATCAACAGAAGCC | Green et al., 2004 |
| ALS5F | TGACTACTTCCAGATTTATGCCGAG | Green et al., 2004 |
| ALS5R | ATTGATACTGGTTATTATCTGAGGGAGAAA | Green et al., 2004 |
| ALS6F | GACTCCACAATCATCTAGTAGCTTGGTTT | Green et al., 2004 |
| ALS6R | CAATTGTCACATCATCTTTTGTTGC | Green et al., 2004 |
| ALS7F | GAAGAGAACTAGCGTTTGGTCTAGTTGT | Green et al., 2004 |
| ALS7R | TGGCATACTCCAATCATTTATTTCA | Green et al., 2004 |
| ALS9F | CCATATTCAGAAACAAAGGGTTC | Green et al., 2004 |
| ALS9R | AACTGAAACTGCTGGATTTGG | Green et al., 2004 |
| ORF19.251F | GTCATGGTCCTGCCATTTTT | This study |
| ORF19.251R | AGCACCTTCCTTTTCAGCAA | This study |
| ORF19.4477F | TGGTGTTTTGTGTCGTCCAT | This study |
| ORF19.4477R | CACCATGCCAATGAAACTTG | This study |
| ORF19.7310F | TCAGCAACAAGGTTGACGAG | This study |
| ORF19.7310R | GAGTAGCCTGAGACCCAACG | This study |
| ORF19.1862F | ACAACGTGGTGCTGAAGGTA | This study |
| ORF19.1862R | GTGGGATCCACCAATTTCAC | This study |
| ORF19.5760F | TGATGCCTCAGGTACTGCTG | This study |
| ORF19.5760R | CCTGAAGCAGCAGTTGTCAT | This study |
| ORF19.3160F | AAAGGGCAAGGAACAAGTCA | This study |
| ORF19.3160R | TTCAGCAGCCTTTCCAATTT | This study |
| ORF19.3548.1F | TTGGTGATAAAATCGAATCCAA | This study |
| ORF19.3548.1R | GCATCAGAAGCTTTTTGGACA | This study |
| ORF19.2531F | TGAAAAATGACGCCGATAAA | This study |
| ORF19.2531R | TCGTAATGTTTGTGCCATTCA | This study |
| ORF19.4216F | AAAGGGCAAGGAACAAGTCA | This study |
| ORF19.4216R | TTCAGCAGCCTTTCCAATTT | This study |
| ORF19.2849F | TTGCTAACCAAGACCCAACC | This study |
| ORF19.2849R | AATTGGTGGGACAGCTTGAG | This study |
| ECM331F | CCAAACTTGAAATCCGTTGG | This study |
| ECM331R | TGGACCAGAAATGGTGACAG | This study |
| AQY1F | TTGCTAACCAAGACCCAACC | This study |
| AQY1R | AATTGGTGGGACAGCTTGAG | This study |
| HSP12-O/EXP-F | GTGATAATAAGTGTGAAGAGTAGTAATTGAAGTTTGGTGTGTCTAGGTGGTGTTTAAGACTGTTTAGTATTTTCGCAAGAATTTTGTTAACTTTGTGTGTATCAAGCTTGCCTCGTCCCC | This study |
| HSP12-O/EXP-R | TGGTGTTTGTCGAAAAAAAACAAGATTCAAAGAATTTACCTCTACTAGAGAAATTGACTCTATTTATATTGTTTTTCGAGAGGGGGGCAAAGGTTGCCATATTTGAATTCAATTGTGATG | This study |
| ORF19.7310-O/EXP-F | CCTTGGTTTTCGGACATGAAAATTGATACTAATAAGTGTGTTTTTATTACCAAACCCAGTAAAAGTTGCAAATACAATTCTCATCAAAGTTTCTTTGAGAATCAAGCTTGCCTCGTCCCC | This study |
| ORF19.7310-O/EXP-R | AAAATTGTTTTAAATCATCATTTGTCCATTGATCAAAACCATAATTGGCAACCACAAAAGTGATAGATACATATAACCATACAATGATTGGGAAATACATATTTGAATTCAATTGTGATG | This study |
| IHD1-O/EXP-F | CTATTAAATTGACCCCATTACTACATAGATTTTTAGTTCATAGTCTTTATGGTTTGCTTTGTTTCTTTAATCGCATTGATAATTCAATATTTGTGTTACAATCAAGCTTGCCTCGTCCCC | This study |
| IHD1-O/EXP-R | TTTGGTATGCGTTAGAACAGTGGTCGTTACAAATTTGGTCAGCTCTTTTTGCGGCAAGAGCAATTTGTAAAAGTGCAAAGAATAATGGAGTTGGTCTCATATTTGAATTCAATTGTGATG | This study |
| ORF19.251-O/EXP-F | GCGTTGTTATATTGTTGATGAATCGAAGAAAACTAGATTACTAATTGAAATGGTACAAAATAGTTCAACCGTTTCAAGTTTTCAATTATCAGAATTTTCGATCAAGCTTGCCTCGTCCCC | This study |
| ORF19.251-O/EXP-R | CAAAAGGATGTAAAGCTTCAACAACGAAAACACCAGTCTTTTTACCGTCACTGTAAAAAGTTTCGTTATAAGAAGTAAGAGCGAGTAAAACTTTGACCATATTTGAATTCAATTGTGATG | This study |
| AQY1-O/EXP-F | TATACTTTCTAGAAATAAGAAAAGGGTTACCCTATCACTATTCTTCTCTTTGGTTAGTTACCAAAACCAAATTCTACTTCTTGGACAACTTCTTTTCCCT ATCAAGCTTGCCTCGTCCCC | This study |
| AQY1-O/EXP-R | GCGACACATTGACAGAATCGTCATATTTTGGTTCATAGACTGGTCTTTGAGCCTCAACGTCGTTAGGAGTATTATCAATGCTAGAACTTTCGGCAACCAT ATTTGAATTCAATTGTGATG | This study |
| ORF19.1862-O/EXP-F | TTGTGGATCCAATACAGCTATTGTAGTGGTACTTACTGAAATGTAATCACTTCGCATTATTCAAGATCTTTTTCTATTGTGGCTGGTTTTTGGTGATTGC ATCAAGCTTGCCTCGTCCCC | This study |
| ORF19.1862-O/EXP-R | TTATATTTGGCTAACAAATCGGCATCATCGATGAAAATGACAAAATCGTTTTCAGCACCTTTGTAGAATAATTTATGTGGAGAAGTTTTAGCCATATTTGAATTCAATTGTGATG | This study |
| ORF19.4216-O/EXP-F | AAACAGCCTAGTGATAATAAGTGTGAAGAGTAGTAATTGAAGTTTGGTGTGTCTAGGTGGTGTTTAAGACTGTTTAGTATTTTCGCAAGAATTTTGTTAACT ATCAAGCTTGCCTCGTCCCC | This study |
| ORF19.4216-O/EXP-R | TTATGGTGTATGTCGAAAAAAAACAAGATTCAAAGAATTTACCTCTACTAGAGAAATTGACTCTATTTATATTGTTTTTCGAGAGGGGGGCAAAGGTTGCCAT ATTTGAATTCAATTGTGATG | This study |
| CSP37-O/EXP-F | GAATAGTCACAGAGAGAGAGAGAAGGATTGCTATTTTTATTTTTTTGGGGGCATAATCACTTATTATACGTCACCACCACCACTAATGGATATATGATTC ATCAAGCTTGCCTCGTCCCC | This study |
| CSP37-O/EXP-R | GGTGTTGTTGTCTTGGTAAGATTGGTTGAACATATTGATCATAATAATAAACCCCACCGACAAGGGCAGCAGTACCTAATAAATATTTTCCAGCAGACATATTTGAATTCAATTGTGATG | This study |
| NAT-OE-R-DET | GAA ACA ACA ACG AAA CCA GC | Nobile et al., 2006 |
| ALS2-F-O/EXP | ACTTCTTCAAATACTAAAACGTTTTCACATAAACTGCAAGCCTCAAATTGATAGTATTCTACAGTATAAATGCTGAACTAACTTATTTTTACAATTACAA ATCAAGCTTGCCTCGTCCCC | This study |
| ALS2-R-O/EXP | TTGTCCATGTCAACGAATCAAAACTATTGAAAACACCCGTAATAACTTTTGCAGTAGCAACTGATACACAGAGGCTTAGCAACAAAAATTGTAAAAGCAT ATTTGAATTCAATTGTGATG | This study |
| ALS4-F-O/EXP | TTGATAGTTCTCTCCAGTATAAATGCTGAATTAACTCATTTTTACAATTACAAATTAGTAATTATGCATTTGAAAATGGTGCTTACGTTTGATTTCAATG ATCAAGCTTGCCTCGTCCCC | This study |
| ALS4-R-O/EXP | TGGTCCAAGTTAACGAATTAAAACTATCGAAAATGCCCGTAATAACCTTTGCCGTAGCAACTGATACACAGAGGCTTAGCAACAAAAATTGTAAAAGCAT ATTTGAATTCAATTGTGATG | This study |
| ALS1-O/EXP-F | GAATCTGCAATGAAAACGTTAAGACATTGGAATTTTTCATCAAATTTACGATGAATTGCTAATCATCTTTGGAGATATTCGTAGTAAGATCTTCAACCCA ATCAAGCTTGCCTCGTCCCC | This study |
| ALS1-O/EXP-R | TGGACCAAGTTAATGAATTAAAACTATCAAAAACACCAGTGATTGTCTTTGCACTTGCAATTGACAAATATAGGAATAACAATGTAAATTGTTGAAGCAT ATTTGAATTCAATTGTGATG | This study |
| WH11-O/EXP-F | CTAAATTTCTTCTTATTTCTACATTTAAGGGAACACCCTATTCAACCTATGGAATTGTGATTTCACCTTGAAATCCTCGGGATCTGCATAAACTAAGTTG ATCAAGCTTGCCTCGTCCCC | This study |
| WH11-O/EXP-R | TAACCTTATCCTTGAATTGTTCTGGAGTGGATTTTTGAGAATCTGGAGTTAATTTGGATTCGATTTTATCACCAATATCTTTTCTACCTAAGTCGGACAT ATTTGAATTCAATTGTGATG | This study |
| CSH1-F-O/EXP | ACGGAATGCCATCAACTGTTGTGAGAAACTTGGGACTATAGTATAGAGAACATTCAAGCTTGGTGTTGTTATAGATGATTTGATTAATAATGTAAAAAAT ATCAAGCTTGCCTCGTCCCC | This study |
| CSH1-R-O/EXP | CGATGTCACCATTAAAACCTCTCCAACTGGATCCCAATCTCATAGTACCGACAGCAACAGTGTTGACCTTCAAACCAGATTTACCTAATCTGGTGACCAT ATTTGAATTCAATTGTGATG | This study |
| ECM331-F-O/EXP | GGTGGTAGTAATGACAATTTGATAGTATTGTTACTTTTCGTGTTTTCTAACTCATTTATACAACATTCCTTTTGTTGCATTACTTTACTATAGCAACAAT ATCAAGCTTGCCTCGTCCCC | This study |
| ECM331-R-O/EXP | TGGAAGTTTTAGAGAATGAACATTTGTTTGATGAGTCTGCTGCTGAAACTGATGTCAATAAGGCAGCGACTATTGGTAAAAGAAATGACTTAATTTGCAT ATTTGAATTCAATTGTGATG | This study |
| TPK1-SAT1-F1 | ACTGTCACGCAA GGTACCAAGTTTTAACTAACTA | This study |
| TPK1-SAT1-R1 | AACTTCTCGAGCAGAAACAGTGTTGAATAATATCACCTT | This study |
| TPK1-SAT1-F2 | TAATTAATTTGCCCGCGGAAGAAAGAACAAGAAA | This study |
| TPK1-SAT1-R2 | AGACTTGACAAACGAGCTCTTTAGTATCACCGTA | This study |