Fig. 2.

Effects of treatment with sesame constituents on reporter gene activity in DLD-1/COX-2-B2-β-gal-BSD cells. DLD-1/COX-2-B2-β-gal-BSD cells were seeded in 96-well multiwell plates at a density of 2 × 10⁵ cell/well and cultured in medium containing sesame constituents at concentrations up to 100 µM. After 48 h, the COX-2 promoter activity was evaluated by β-galactosidase activity and was normalized for viable cell numbers assessed by MTT assay. The columns indicate the values of the mean percentages of triplicate wells of promoter activity of DLD-1/COX-2-B2-β-gal-BSD cells. The data are representative of more than three independent experiments. Bars indicate the SD. *p<0.05. (A), sesamol; (B), sesamin; (C), sesamolin; (D), ferulic acid; (E), syringic acid. COX; cyclooxygenase, cPGES; cytosolic PGES, mPGES; microsomal PGES, PGES; prostaglandin E synthase.