Fig. 5.

Prostaglandin E₂ receptor expression in human colorectal cancer cells with or without sesamol treatment. DLD-1/COX-2-B2-β-gal-BSD cells were seeded in 6-well multiwell plates at a density of 2 × 10⁶ cell/well and cultured in medium containing 50 and 100 µM sesamol for 48 h. After 48 h treatment, quantitative real-time PCR analysis was performed to determine EP1(A), EP2(B), EP4(C) mRNA levels. Data are normalized with GAPDH, Data are mean ± SD, n = 3.**p<0.001 vs 0 ppm. (D) EP1–4 protein was detected by western blot analysis, using DLD-1/COX-2-B2-β-gal-BSD cells (24-well plates at a density of 2 × 10⁵ cell/well) with treatment of 50 and 100 µM sesamol for 24 and 48 h.