FIGURE 1.

NK frequencies and numbers are reduced in E4BP4-deficient mice. The wt and E4BP4^−/− thymi (A), spleens (B), and livers (C) were analyzed for the presence of CD3⁻NK1.1⁺NKp46⁺CD122⁺ NK cells. Absolute numbers of total CD3⁻NK1.1⁺NKp46⁺CD122 NK cells were then calculated based on the gating strategy shown. Bars in the graph show means ± SD of replicates (n = 8–14); the fold reduction is indicated to the right. Asterisks indicate statistically significant differences (unpaired t test: **p < 0.01).