Figure 5.

PAR-3 knockdown delays tight junction complex reassembling after 1 mol/L mannitol treatment. HCMEC/D3 cells treated with control (A), claudin-5 (cldn-5) (B) or PAR-3 (C) small interfering RNAs were grown on a 96-well E-plate. Impedance measurement was monitored in real time by xCELLigence system and curves were generated using the RTCA Software. After 72 hours, cells were treated with 1 mol/L mannitol. After another 30 minutes, medium was changed to normal growth medium for a recovery period of up to 4 days. Black curve: untreated cells; light gray curve: 1 mol/L mannitol-treated cells. Results are mean cell index (CI) values±s.d. (n=3 wells) from one representative experiment out of three independent experiments.