Figure 1.

Cell differentiation and proliferation in vitro. (A) Neural stem cells (NSCs) were stained with the neuronal marker mitogen-activated protein 2 (MAP2) (red), glial fibrillary acidic protein (GFAP) (red), the oligodendrocytic marker NG2 (red), and the immature cell marker Nestin (red), and 4′,6 diamidino-2-phenylindole (blue). The NSCs differentiated into neurons, astrocytes, and oligodendrocytes. Some cells were still stained with Nestin. Scale bars, 50 μm. (B) Fluorescent staining with the proliferation marker Ki-67 (red), green fluorescent protein (green), and 4′,6 diamidino-2-phenylindole (blue) after culturing in differentiation medium. Scale bar, 50 μm. WT, wild-type; Tg, transgenic.