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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: J Immunol. 2014 Jan 10;192(4):1609–1619. doi: 10.4049/jimmunol.1301599

FIGURE 2.

FIGURE 2

A pre-assembled TCRβ gene completely rescues early αβ T cell development in Rag1C/C mice. A. Representative flow cytometry analysis of CD4 and CD8 expression on total thymocytes isolated from littermate or age-matched WT (n=3), Rag1C/C (n=3), Jβ1DJ/DJ (n=3), and Rag1C/CJβ1DJ/DJ mice (n=3). The average number of total thymocytes for each genotype is indicated in parentheses, and the frequencies of cells in the DN, DP, CD4+ SP, and CD8+ SP quadrants are indicated on the plots. B. Graphs showing the average numbers of DN and DP thymocytes from mice of the indicated genotypes. Error bars are standard error of the mean. Lines with asterisks above indicate significant differences (p≤0.05). A and B. This experiment was independently performed three times, each time on one mouse of each genotype. C. Representative flow cytometry analysis of c-Kit and CD25 expression on DN thymocytes isolated from littermate or age-matched WT (n=3), Rag1C/C (n=3), Jβ1DJ/DJ (n=3), and Rag1C/CJβ1DJ/DJ (n=3) mice. The frequencies of DN cells in the DN1, DN2, DN3, and DN4 quadrants are indicated. D. Graphs showing the average numbers of DN3 and DN4 thymocytes from mice of the indicated genotypes. Error bars are standard error of the mean. Lines with asterisks above indicate significant differences (p≤0.05). C and D. This experiment was independently performed three times, each time on one mouse of each genotype. E. Representative flow cytometry analysis of CD4 and CD8 expression on total thymocytes isolated from littermate or age-matched WT (n=3), Rag1C/C (n=3), Vβ1NT/NT (n=6), and Vβ1NT/NTRag1C/C (n=6) mice. The average number of total thymocytes for each genotype is indicated in parentheses, and the frequencies of cells in the DN, DP, CD4+ SP, and CD8+ SP quadrants are indicated on the plots. F. Graphs showing the average numbers of DN and DP thymocytes from mice of the indicated genotypes. Error bars are standard error of the mean. The line with an asterisk above indicates a significant difference (p≤0.05). E and F. This experiment was independently performed three times, each time on at least mouse of each genotype. G. Representative flow cytometry analysis of c-Kit and CD25 expression on DN thymocytes isolated from littermate or age-matched WT (n=3), Rag1C/C (n=3), Vβ1NT/NT (n=3), and Vβ1NT/NTRag1C/C (n=3) mice. The frequencies of DN cells in the DN1, DN2, DN3, and DN4 quadrants are indicated. H. Graphs showing the average numbers of DN3 and DN4 thymocytes from mice of the indicated genotypes. Error bars are standard error of the mean. Lines with asterisks above indicate significant differences (p≤0.05). G and H. This experiment was independently performed three times, each time on one mouse of each genotype.