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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: J Immunol. 2014 Jan 10;192(4):1609–1619. doi: 10.4049/jimmunol.1301599

FIGURE 6.

FIGURE 6

Replacement of the Vβ14 RSS with the more efficient 3’Dβ1 RSS increases Vβ14 recombination frequency and rescues αβ T cell development in Rag1C/C mice.A. Representative flow cytometry analysis of TCRβ and Vβ14 expression on total thymocytes isolated from littermate or age-matched Vβ143’Dβ1RSS/+ (n=3) and Rag1C/CVβ143’Dβ1RSS/+ (n=3) mice. The frequencies of cells in the depicted TCRβintermediate gates are indicated. B. Graph showing the average frequencies of TCRβintermediate cells expressing Vβ14 in thymocytes from mice of the indicated genotypes. Significant differences of *p<0.05 indicated. A and B. This experiment was independently performed three times, each time on at least one mouse of each genotype. C. Representative flow cytometry analysis of CD4 and CD8 expression on total thymocytes isolated from littermate or age-matched Vβ143’Dβ1RSS/+ (n=3) and Rag1C/CVβ143’Dβ1RSS/+ (n=3) mice. The average number of total thymocytes for each genotype is indicated in parentheses, and the frequencies of DN, DP, CD4+ SP, and CD8+ SP cell populations are indicated on the plots. D. Graph showing the average number of DN and DP cells from mice of the indicated genotypes. Error bars are standard error of the mean. Significant differences of *p<0.05 indicated. C and D. This experiment was independently performed three times, each time on at least one mouse of each genotype. E. Representative flow cytometry analysis of c-Kit and CD25 expression on DN thymocytes isolated from littermate or age-matched Vβ143’Dβ1RSS/+ (n=3) and Rag1C/CVβ143’Dβ1RSS/+ (n=3) mice with the frequencies of DN1, DN2, DN3, and DN4 cell populations indicated. F. Graph showing the average numbers of DN3 and DN4 cells from mice of the indicated genotypes. Error bars are standard error of the mean. Significant differences of *p<0.05 indicated. This experiment was independently performed three times on a total of three mice of each genotype.C and D. This experiment was independently performed three times, each time on at least one mouse of each genotype.