Table 1.
Primary antibodies used in this study
| Antibody | Type | Specificity | Phosphorylation sites |
Source / Reference |
|---|---|---|---|---|
| GFAP | Mono- | GFAP | Millipore, Temecula, CA, USA | |
| Iba1 | Poly- | Iba1 | Wako Chemicals, Richmond, VA, USA |
|
| Synaptophysin | Mono- | Synaptophysin | Millipore | |
| PSD95 | Mono- | PSD95 | Cell signaling Technology, Danvers, MA, USA |
|
| GluR1 | Poly- | GluR1 | Millipore | |
| GluR2/3 | Mono- | GluR2/3 | Abcam, Cambridge, MA, USA | |
| NR1 | Poly- | NR1 | Thermo Scientific, Pittsburg, PA, USA |
|
| IRβ | Poly- | IRβ | Cell signaling Technology | |
| IGF-1Rβ | Poly- | IGF-1Rβ | Cell signaling Technology | |
| P-IRβ/IGF -1Rβ | Mono- | P-IRβ/IGF -1Rβ | Tyr1150/1151(IRβ), Tyr1135 /1136 (IGF-1Rβ) |
Cell signaling Technology |
| IRS1 | Poly- | IRS1 | Cell signaling Technology | |
| IRS1 pS307 | Poly- | P-IRS1 | Ser307 | Cell Signaling Technology |
| PI3K p85 | Poly- | PI3K (p85) | Cell Signaling Technology | |
| P-PI3K p85 | Poly- | P-PI3K (p85) | Tyr458/Tyr199 | Cell Signaling Technology |
| PDK1 | Poly- | PDK1 | Cell Signaling Technology | |
| PDK1 pS241 | Poly- | P-PDK1 | Ser241 | Cell Signaling Technology |
| AKT | Poly- | AKT | Cell Signaling Technology | |
| AKT pS473 | Poly- | P-AKT | Ser473 | Cell Signaling Technology |
| AKT pT308 | Poly- | P-AKT | Thr308 | Cell Signaling Technology |
| GSK-3α/β pS21/9 | Poly- | P-GSK-3α/β | Ser21/9 | Cell Signaling Technology |
| GSK-3α/β | Poly- | GSK-3β | Cell Signaling Technology | |
| R134d | Poly- | Tau | [70] | |
| pS199/202 | Poly- | P-tau | Ser199/2002 | Invitrogen, Grand Island, NY, USA |
| pT205 | Poly- | P-tau | Thr205 | Invitrogen |
| pT212 | Poly- | P-tau | Thr212 | Invitrogen |
| pS214 | Poly- | P-tau | Ser214 | Invitrogen |
| pT231 | Poly- | P-tau | Thr231 | Invitrogen |
| 12E8 | Mono- | P-tau | Ser262/356 | Dr. D. Schenk |
| PHF-1 | Mono- | P-tau | Ser396/404 | Dr. P. Davies |
| pS404 | Poly- | P-tau | Ser404 | Invitrogen |
| pS422 (R145) | Poly- | P-tau | Ser422 | [71] |
| Anti-GAPDH | Poly- | GAPDH | Santa Cruz Biotechnology | |
| 6E10 | Mono- | APP and Aβ | Covance, Princeton, NJ, USA |