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RifDH10-catalyzed dehydration/hydration of RifACP10-bound substrates a) Dehydration of (2S,3S)-2-methyl-3-hydroxypentanoyl-RifACP10 (1a) b) Hydration of (E)-2-methyl-2-pentenoyl-RifACP10 (2). c) Incubation of Rif[DH10+KR10] with RifACP10- and EryACP6-bound 2-methyl-3-ketoacyl thioesters.
