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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: Biochemistry. 2013 Nov 25;52(49):8916–8928. doi: 10.1021/bi400988t

Figure 4.

Figure 4

Chiral GC-MS analysis of the incubation of (2RS)-2-methyl-3-ketopentanoyl-RifACP10-NusA with RifDH10-KR10 (Methods 2 and 4). Left panels: A1. Extracted ion current (XIC) at m/z 88 (base peak for 4a) (Method 4); B-1 and C-1. Extracted ion current (XIC) at m/z 114 (base peak for 3) (Method 2). Right panels, mass spectra of selected peaks, upper half, observed spectrum, lower half, inverted mass spectrum of reference standard. A. (2S,3S)-4a produced by RifDH10-KR10. B. (E)-2-Methylpentenoic acid (3) produced by RifDH10-KR10.