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RifDH10-catalyzed dehydration/hydration of acyl thioester analogues. a) Dehydration of (2R,3R)-2-methyl-3-hydroxypentanoyl-EryACP6 (5b). b) Hydration of (E)-2-methyl-2-pentenoyl-EryACP6 (6). c) Dehydration of (2R,3R)-2-methyl-3-hydroxypentanoyl-S-Pant (7b) and –S-NAC (9b) analogues. d) Incubation of RifDH10 with (E)-11 and (Z)-13.
