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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Mol Pharm. 2013 Aug 19;10(9):3342–3355. doi: 10.1021/mp4001316

Figure 5. Prior Exposure to Adenovirus by a Route Different from that of Immunization Affords Better Protection from Lethal Challenge than when the Same Route is used for Both Procedures in Mice.

Figure 5

Naïve B10.Br mice and those with prior exposure to adenovirus 5 were challenged with a lethal dose of MA-ZEBOV 28 days after immunization by the IM, IN or SL route. Control, naïve mice were given saline (PBS). Panels A–C. Kaplan–Meier survival curves. In each panel, asterisks indicate a significant difference with respect to naïve, immunized animals. Panels D–F. Body Weight Profiles after Challenge. No significant change in body weight was noted in animals that survived challenge. Panels G–I. Post-challenge serum alanine (ALT) and aspartate (AST) aminotransferase levels. Samples were taken from survivors at the termination of the study and from non-survivors at time of death. Asterisks indicate a significant difference with respect to naïve, immunized animals. Panels J–L. Serum Cytokines Levels After Challenge. Samples were taken from survivors 14 days after challenge and from non-survivors at time of death. In all panels, data reflect average values ± the standard error of the mean for 9–10 mice per group. *p<0.05, **p<0.01, ***p<0.001, one-way ANOVA, Bonferroni/Dunn post-hoc analysis.