Fig. 3.

Effects of PEG-catalase on ADP-dependent modulation of PIP₂, AMPK, and c-Abl. Endothelial cells were transfected with plasmids encoding FRET biosensors specific for PIP₂ (A), AMPK (B), and CrKII (C) and then analyzed by quantitative FRET microscopy before and after the addition of ADP (50 μM, 5 min) in the presence and absence of PEG-catalase. A–C Left show representative photomicrographs; A–C Right show representative tracings of FRET ratios as well as statistical analysis of the ADP-promoted FRET slope change, as pooled and plotted from four independent experiments; *P < 0.05 (ANOVA).