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. 2014 Feb 18;111(9):3377–3382. doi: 10.1073/pnas.1307415111

Fig. 2.

Fig. 2.

Transcription is required for mutations of THO complex components to induce early senescence and formation of type II survivors in the absence of telomerase. (A) hrs1 restores the premature senescence of tlc1 tho2 and tlc1 hpr1 cells. Freshly dissected colonies with the indicated mutations were continuously streaked onto YC plates. (B) The senescence rates were measured in liquid culture by serially passaging spore products of the indicated genotypes. (C) hrs1 delayed type II telomere recombination in tlc1 cells. DNA from the indicated strains was collected, digested by using a combination of AluI, HaeIII, HinfI, and MspI, and blotted with a C1–3A probe. (D) sub2-5 overexpression restores the premature senescence of tlc1 tho2 and tlc1 hpr1 cells. The indicated yeast cells harboring pRS425 or pRS425–SUB2-5 were continuously streaked onto YC plates at 30 °C. (E) sub2-5 overexpression restored the early formation of type II telomere recombination in tlc1 tho2 and tlc1 hpr1 cells. DNA from the indicated strains was then analyzed by Southern blotting using a C1–3A probe.