Fig. 5.

Induction of the DNA damage response and recruitment of recombination protein onto telomeres in THO mutants lacking telomerase. Colonies from fresh-dissected tetrads were inoculated directly into liquid YEPD medium and cultured at 30 °C until the stationary phase. The cultures were diluted and cultured at 30 °C. The procedures were repeated seven to eight times. Total cell extracts were prepared form these cells and immunoblotted with antibodies against Rad53 (Upper) or actin (Lower). Quantifications for the percentage of phosphorylated Rad53 over total Rad53 were plotted.