Skip to main content
. 2014 Feb 18;111(9):3567–3572. doi: 10.1073/pnas.1315156111

Fig. 3.

Fig. 3.

Growth inhibition of TgALD-depleted parasites by glucose or fructose. (A) Depletion of TgALD in the ALD cKO strain. The ALD cKO strain and WT complement (ALD cKO/ALD) were grown in glucose-free medium ±0.5 μg/mL ATc for 36 h before analysis. TgALD was detected using rabbit anti-TgALD. TgMIC2 was detected using mAb 6D10 as a loading control. Primary antibodies were detected as above. Dilutions of the parental TATi line (tet-transactivator) were used to estimate levels of TgALD. (B) Host monolayer lysis by parasite growth for 48 h in media ±4,500 mg/L glucose (containing 0.5 μg/mL ATc). (C) Host monolayer lysis by growth of parasites for 48 h in media (with 0.5 μg/mL ATc) containing glucose or fructose (4 × 105 parasites per well). (B and C) Parasites were pretreated with 0.5 μg/mL ATc for 36 h in glucose-free medium before analysis. Means ± SD combined from two independent experiments (each in triplicate) for B (n = 6) or three for C (n = 9).