Fig. 2.

MLN CD103⁻ DCs are proinflammatory in acute colitis. (A) Representative photomicrographs of H&E colonic sections and histological score from control colitic and mice transferred with 2 × 10⁵ colitic MLN CD103⁻ or CD103⁺ DCs. (Magnification: 20×.) (Scale bars: 100 μm.) (B) [³H] thymidine (TdR) incorporation of CD4⁺ T cells cocultured with colitic DCs (72 h). Results are shown as mean ± SEM of triplicate wells. (C) FACS analysis of cells from B for intracellular cytokine and Foxp3 detection. Numbers of IL-17A⁺, IFN-γ⁺, and Foxp3⁺ CD4⁺ T cells per 10⁵ total cells are shown. Opn levels of MLN homogenates from healthy (h) and colitic (c) mice (D) and relative Spp1 mRNA expression in MLNCs (E) are shown. Values are expressed as mean ± SEM (n = 5–6 mice per group) from three separate experiments. (F) Relative Spp1 mRNA expression in colitic MLN CD103⁻ and CD103⁺ DCs. Values are from one representative experiment (*P < 0.05; **P < 0.01; ***P < 0.0005).