Fig. 3.

BALB/c Spp1^−/− mice are protected from acute colitis. (A) Representative colon photographs, photographs (H&E) of sections, length, histological scores, and differential cell counts of mice with TNBS colitis. HPF, high-power field; LMs, lymphomononuclear cells; Mϕs, macrophages; PMNs, polymorphonuclear leukocytes. (Magnification: 10×.) (Scale bars: 200 μm.) (B) Levels of cytokines in MLN homogenates. (C) Percentages of IL-17A⁺ and IFN-γ⁺ CD4⁺ T-cell MLNCs and numbers of IL-17A⁺, IFN-γ⁺, Foxp3⁺, and IL-10⁺ CD4⁺ T-cell MLNCs. Values are expressed as mean ± SEM (n = 6 mice per group) from five separate experiments. (D) Numbers of DC subpopulations gated as in Fig. 1B. Values are expressed as mean ± SEM (n = 4 mice per group) from two separate experiments (*P < 0.05; **P < 0.005; ***P < 0.001).