Fig. 3.

Metabolic gene expression profiles distinguish two cluster of differential regulation in ccRCC that implicate a role of one-carbon metabolism in the malignancy. (A) Unsupervised hierarchical clustering of ccRCC log₂ gene expression fold-changes vs. matched tumor-adjacent normal samples featuring the set of 1,504 metabolic genes significantly different across all log₂ gene expression fold-changes in cancer vs. matched tumor-adjacent normal samples. (B) Clinical data for each ccRCC sample as ordered by the hierarchical clustering in A. Tumor stage ranges from stage 1 (white) to stage 4 (dark red); age is represented by a gray-scale in which the lowest value is 38 y and highest 90 y; metastatic tumors are depicted in blue. (C) Kaplan-Meier survival curves for the two clusters in A (solid line), for the two clusters in SI Appendix, Fig. S19 defined when featuring significantly changed genes between the pool of high tumor stage and low tumor stage samples (short dashed line), and for the two groups of samples solely identified by a high or low tumor stage (long dashed line). Blue and green lines refer to high and low tumor stage, respectively. (D) Gene expression regulation of enzymes involved in choline degradation to glycine in the two clusters in A.