Table 1. Changes of selection conditions in cell-SELEX process.
| Cycle | Cell density | ssDNA (pmol) | Yeast tRNA (ug/uL) | Salmon sperm DNA (ug/uL) | Incubation time | Washing conditions |
| 1 | 2×106 | 1000 | 0.1 | 0.05 | 60 min | 3×1 min |
| 2 | 1×106 | 800 | 0.1 | 0.1 | 40 min | 3×2 min |
| 3 | 4×105 | 200 | 0.1 | 0.1 | 40 min | 3×3 min |
| 4* | 4×105 | 150 | 0.1 | 0.1 | 40 min | 3×3 min |
| 5* | 4×105 | 150 | 0.5 | 0.1 | 30 min | 4×2 min |
| 6* | 4×105 | 100 | 1 | 1 | 30 min | 4×2 min |
| 7* | 4×105 | 80 | 0.5 | 1 | 30 min | 5×2 min |
| 8* | 4×105 | 100 | 1 | 1 | 30 min | 5×2 min |
| 9* | 2×105 | 100 | 1 | 1 | 30 min | 5×2 min |
| 10* | 2×105 | 100 | 1 | 1 | 30 min | 5×2 min |
| 11* | 2×105 | 100 | 1 | 1 | 30 min | 5×2 min |
*U87MG cells were introduced for counter selection since the 4th round.