Table 4. Relative Immunostaining Intensity of TGF-β Receptors in Wounded WT and FMOD-null Adult Mice.
| After injury | Unwounded | 0.5 day | 1 day | 2 days | 7 days | 14 days | ||||||
| TβRI | WT* | FN* | WT | FN | WT | FN | WT | FN | WT | FN | WT | FN |
| Epidermis | ||||||||||||
| Migrating epi. | N/A* | N/A | −* | − | − | +++* | +++ | +++ | N/A | N/A | N/A | N/A |
| Outer layer | +++ | +++ | − | − | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Basal layer | +++ | +++ | − | − | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Dermis | ||||||||||||
| ECM | − | − | − | − | − | − | − | − | − | − | − | − |
| Fibroblasts | +++ | +++ | − | ++* | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Inflammatory cells† | − | − | − | ++ | +++ | +++ | +++ | +++ | − | − | − | − |
| Hair follicles‡ | +++ | +++ | − | − | +* | + | +++ | +++ | +++ | +++ | +++ | +++ |
* WT, wild-type; FN, FMOD-null; N/A, not applicable; −, negligible staining (<5%); +, minimal staining (5%–25%); ++, moderate staining (25%–50%); +++, strong staining (>50%).
†
In general, unwounded control skin contained very few inflammatory cells. The chart reflects the intensity of intracellular TGF-β receptor staining and not the actually number of inflammatory cells present.
‡
Due to no hair follicle regeneration was detected in the adult skin wounds in this study, the chart reflects the influence of the wounds on adjacent hair follicles.