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. 2014 Mar 6;9(3):e90500. doi: 10.1371/journal.pone.0090500

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© 2014 Bao, Schild

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Dots and asterisks indicate the computational cost in Legendre - and time - domain, respectively, on a logarithmic scale. Black and red curves refer to whether (red) or not (black) a system response function was taken into account. Each point or asterisk is the average duration of computations. As the analysis in the time - domain is fastest when using the Fast Fourier Transform, we choose the sample sizes to be powers of , starting with . Inset, left, FLIM image of a mouse hippocampus neuron probed for oxidative stress using the hydrogen peroxide sensor HyPer [13] and, right, fluorescence lifetime function for one pixel in the middle of the cell (150 samples, data courtesy K. Kizina and M. Müller, CNMPB, Göttingen).

Inline graphic — Dots and asterisks indicate the computational cost in Legendre - and time - domain, respectively, on a logarithmic scale. Black and red curves refer to whether (red) or not (black) a system response function was taken into account. Each point or asterisk is the average duration of computations. As the analysis in the time - domain is fastest when using the Fast Fourier Transform, we choose the sample sizes to be powers of , starting with . Inset, left, FLIM image of a mouse hippocampus neuron probed for oxidative stress using the hydrogen peroxide sensor HyPer [13] and, right, fluorescence lifetime function for one pixel in the middle of the cell (150 samples, data courtesy K. Kizina and M. Müller, CNMPB, Göttingen).