Table 4. List of glycosyl hydrolases (GHs) in Anoxybacillus and their similarity to the other genera.

GH	Enzyme	Similarity within Anoxybacillus a					Sequence similarity to other genera (%)
		SK3-4	DT3-1	WK1	TNO-09.006	G10
1	β-glucosidase	-	92.4	100	-	-	β-glucosidase from Thermoanaerobacter pseudoethanolicus ATCC 33223, 57%
	β-glucosidase	100	-	-	-	-	β-glucosidase from Geobacillus kaustophilus and Geobacillus thermoleovorans, 98%
2	Glycoside hydrolase	-	-	-	100	-	Glycoside hydrolase from Geobacillus sp. Y412MC52, 98%
13	Amylopullulanase	88.8	-	100	-	99.1	Amylopullulanase from Geobacillus stearothermophilus TS-23, 83%
	Pullulanase	90.5	89.5	100	95.6	89.4	Pullulanase from Geobacillus thermoglucosidasius, 59%
	α-amylase (cell-bound)	97.4	96.2	100	97.2	96.2	α-amylase from Geobacillus sp. WCH70, 73%
	α-amylase (extracellular)	100	-	-	-	95.0	α-amylase Geobacillus stearothermophilus TS-23, 94%
	Cyclomaltodextrinase	92.5	92.0	100	94.7	92.3	Maltogenic amylase from Geobacillus stearothermophilus, 74%
	Glycosidase	96.4	96.6	100	97.8	96.2	Oligo-1,6-glucosidase from Geobacillus thermoglucosidasius, 85%
	Oligo-1,4-1,6-alpha-glucosidase	96.0	96.0	100	98.1	96.0	Oligo-1,6-glucosidase from Geobacillus thermoglucosidasius, 71%
	Oligo-1,4-1,6-alpha-glucosidase	95.2	96.3	100	95.4	96.1	α-amylase catalytic region from Geobacillus sp. Y4.1MC1, 82%
	Trehalose-6-phosphate hydrolase	94.2	93.7	100	-	93.9	α-phosphotrehalase from Geobacillus sp. WCH70, 82%
	1,4-α-glucan branching enzyme	93.2	92.8	100	94.1	92.4	1,4-α glucan branching enzyme from Geobacillus stearothermophilus, 77%
23	Lytic murein transglycosylase	93.6	85.8	100	96.1	85.8	Lytic transglycosylase, Geobacillus sp. WCH70, 60%
31	α-glucosidase	91.0	89.2	100	88.1	89.2	α-glucosidase from Bacillus thermoamyloliquefaciens, 60%
32	Sucrase-6-phosphate hydrolase	91.3	91.8	100	91.0	91.3	β-fructosidase from Bacillus megaterium, 53%
	Sucrose-6-phosphate hydrolase	-	-	100	-	-	Sucrose-6-phosphate hydrolase from Geobacillus sp. Y4.1MC1, 64%
36	α-galactosidase	-	-	-	-	100	α-galactosidase from Geobacillus stearothermophilus, 81%
43	Glycosyl hydrolase	-	-	100	-	-	Putative exo-xylanase from Geobacillus thermoleovorans, 83%
51	α-L-arabinofuranosidase	-	-	100	-	-	α-L-arabinofuranosidase from Geobacillus thermoleovorans, 91%
52	β-xylosidase	-	-	-	-	100	β-xylosidase from Geobacillus thermoglucosidasius, 92%
65	Sugar hydrolase/phosphorylase	94.9	94.1	100	-	94.0	Kojibiose phosphorylase from Bacillus thuringiensis, 61%;
74	Glycosyl hydrolase BNR repeat-containing protein	-	100	-	97.0	-	Glycosyl hydrolase BNR repeat-containing protein from Geobacillus thermoleovorans, 89%

^aThe reference used in the protein sequence alignment is denoted as 100%.