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A, representative immunoblots of NCC, p-T60 NCC, β-ENaC, ROMK, WNK4, SPAK, Sgk1 and actin in kidney cortex after 10 days of experimental diet (20 μg per lane, except for actin blot: 2 μg per lane). B, renal protein levels shown as the mean ± s.e.m. The band densities of the lower β-ENaC lane (∼90 kDa) and the upper SPAK lane (∼75 kDa) were included in calculations of protein abundance (mean ± s.e.m; n = 8–9 each diet; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA).
