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. 2014 Feb 13;592(Pt 5):1139–1157. doi: 10.1113/jphysiol.2013.266924

Figure 8.

Figure 8

NCC, p-T60 NCC, and SPAK protein levels were significantly increased in the low K+ group independent of treatment. A, representative immunoblots of NCC, p-T60 NCC, WNK4, SPAK, and actin in kidney cortex (20 μg per lane, except for actin blot: 2 μg per lane). B, renal protein levels shown as the mean ± s.e.m. The band density of the upper SPAK lane (∼75 kDa) was included in the calculation of protein abundance. Mice received experimental diets for 10 days. Losartan treatment started 1 day prior to the experimental diet and was continued for 11 days (mean ± s.e.m; n = 8–9 each diet; **P < 0.01, ***P < 0.001, one-way ANOVA).