Figure 1.

The Avicel regulons of N. crassa and A. nidulans. (A) Photograph of N. crassa grown on a Petri dish showing mycelia and conidia (orange). (B) Photograph of A. nidulans grown on a Petri dish showing mycelia and conidia (green). (C) Relative mRNA abundance of N. crassa exposed to Avicel versus no carbon conditions. Genes showing significant differential expression are colored by adjusted P-value. (D) Relative mRNA abundance of A. nidulans exposed to Avicel versus no carbon source conditions. Genes showing significant differential expression are colored by adjusted P-value. (E) Total expression from RNA-seq data of genes encoding the major classes of plant cell wall carbohydrate-active enzymes (CAZy; (Cantarel et al. 2009)) from a WT N. crassa culture shifted to media with no carbon source or to Avicel and from a WT A. nidulans culture shifted to media with no carbon source or to Avicel. FPKM (Fragments Per Kilobase per exon per Megabase mapped) for individual genes were averaged between biological replicates and pooled by CAZy class. Classes acting primarily on crystalline cellulose are shown in blue, classes acting primarily on hemicellulose are shown in brown and orange, and classes acting primarily on pectin are colored in purple.