Skip to main content
. 2013 Jun 14;2(4):595–609. doi: 10.1002/mbo3.94

Figure 6.

Figure 6

Phenotypes associated with misexpression of clrB or clr-2 in A. nidulans. (A) Localization of GFP-tagged constructs in cells germinated in liquid glucose medium. GFP-labeled ClrB under native promoter (strain GFP-B) had no detectable fluorescence. GFP-labeled ClrB or CLR-2 driven by the gpdA promoter (strain GFP-PgB and GFP-Pg2) accumulated in nuclei, which were clearly distinguishable by their shape and prominent nucleoli. Arrowheads indicate nuclei. (B) WT and misexpression strains grown 3 days on 1% glucose or 1% ethanol plates at 30°C. PgB and Pg2: constitutive expression of clrB and clr-2, respectively, by the gpdA promoter in ΔclrB background. PaB and Pa2: ethanol-inducible expression of clrB and clr-2, respectively, by the alcA promoter in ΔclrB background. (C) Transcript abundance of two major cellulases (cbhD and cdh) in A. nidulansWT versus strains with constitutive expression of clrB (PgB) or clr-2 (Pg2) when shifted to glucose [G], no carbon media [NC], or Avicel [A] for 6 h. Transcript abundance relative to actA was measured with real-time quantitative RT-PCR. (D) A time course of CMCase activity in WT, ΔclrB, and PgB strains following a shift to either Avicel or sucrose.