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ORFs 28 and 29 constitute a RelBE toxin–antitoxin system, whereas ORF26 is a lone toxin. Serial dilutions of the DJ624Δara containing the pBAD33lev control vector or its derivatives with ORFs 24, 26, or relE (ORF28) and the control vector pKK223-3lev or its derivatives with ORF25, ORF27, or relB (ORF29) were plated on M9 minimal media containing arabinose (1%) and IPTG (0,1 mmol/L) and the appropriate antibiotics. Plates were incubated overnight at 37°C.
