Table 1.
Strains and plasmids used for this study
| Strain or plasmid | Genotype or plasmid properties | Reference or source |
|---|---|---|
| Yeast | ||
| 23344c | MAT ura3 | Our laboratory |
| Bacterial strains | ||
| MC1061 | araD139,D(lacI POZY)74, galU galK rpsL, Δ(araA BC-leu)7697, hsdR mcrB, Sms | Casadaban and Cohen (1980) |
| MG1655 | Wild-type E. coli K-12 | Xiao et al. (1991) |
| DJ624 Δara | MG1655 Δlac malP::lacIq | Our laboratory |
| Plasmids | ||
| pBAD33 | P15A, CamR, pBAD promoter | Guzman et al. (1995) |
| pBAD33lev | pBAD33lev derivative containing URA3 selection gene and a yeast replication origin | This work |
| pBADlev-relE93 | pBAD33lev derivative containing the gene relE93 under the pBAD promoter | This work |
| pBADlev-ORF24 | pBAD33lev derivative containing the ORF24 under the pBAD promoter | This work |
| pBADlev-ORF26 | pBAD33lev derivative containing the ORF26 under the pBAD promoter | This work |
| pKK223-3lev | ColE1, AmpR, pTac promoter | Brosius and Holy (1984) |
| pKK223-3lev-relB93 | pKK223-3lev derivative containing the relB93 gene under the control of the pTac promoter | This work |
| pKK223-3lev-ORF25 | pKK223-3lev derivative containing ORF25 under the control of the pTac promoter | This work |
| pKK223-3lev-ORF27 | pKK223-3lev derivative containing ORF27 under the control of the pTac promoter | This work |