Table 2.

Oligonucleotides used in this study, 40 bp regions homologous to the pBAD33lev and pKK223-3lev required for in vivo recombination yeast are underlined

Primer	Sequence (5′-3′)
ORF24-for-Bad-lev-xba1	TTTTTGGGCTAGCGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAAGGAGGgagtaATGAAAAATGAAATAATCGAC
ORF24-rev-Bad-lev-pst1	AAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGCATGCCTGCAGTTATTTAATCGCATCACTGAG
ORF25-for-pkk-lev-ecoR1	TGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGAATTCATGAAAACTAAAGCACTTTTTG
ORF25-rev-pkk-lev-pst1	CTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGGCTGCAGTCATTGTAAATTGTATTCTTC
ORF26-for-Bad-lev-xba1	TTTTTGGGCTAGCGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAAGGAGGgagtaATGGCAAATTTAGAACCATTC
ORF26-rev-Bad-lev-pst1	AAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGCATGCCTGCAGTTATCTTCTACCTCTTTGACC
ORF27-for-pkk-lev-ecoR1	TGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGAATTCATGTCAAAATGGATTGATAA
ORF27-rev-pkk-lev-pst1	CTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGGCTGCAGCTATTTATCGTTGTTGCTC
relB93-for-lev-ecoR1	TGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGAATTCATGAAAACAGAAAACCCCGT
relB93-rev-lev-pst1	CTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGGCTGCAGTTAGAGCCATTTGGTTAAAAC
relE93-for-lev-xba1	TTTTTGGGCTAGCGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAAGGAGGgagtaATGGCTCTAAATATCCTGTG
relE93-rev-lev-pst1	AAATCTTCTCTCATCCGCCAAAACAGCCAAGCTTGCATGCCTGCAGTCAATAATGACTGCGGATATTC

Primers used to clone into the pBAD33lev vector also include a RBS sequence (AGGAGG) and a spacer sequence (GAGTA) as indicated in bold.