Table 1.
Bacterial strains, plasmids, and primers used in this work
| Strain, plasmid, or primer | Relevant genotype and description, or sequence | Reference |
|---|---|---|
| Strains | ||
| Marinomonas mediterranea | ||
| MMB-1R, wild type | MMB-1, Rifr spontaneous | Solano et al. (2000) |
| LD, lodAB mutant | MMB-1R, ΔlodAB | Gomez et al. (2010) |
| LGD, lodAB, and goxAB mutant | LD, ΔgoxAB | This study |
| LGDAB | LGD Ω mini-Tn10 Kmr (goxAB) | This study |
| Escherichia coli | ||
| UM202 | MP180 katG::Tn10 | Loewen et al. (1985) |
| S17-1 (λpir) | thi pro hsd (r−m+) recA::RP4-2-Tcr::Mu Kmr::Tn7 Tpr Smr, lysogenized by λpir phage | de Lorenzo and Timmis (1994) |
| Plasmids | ||
| pEX18Gm | Gmr;oriT+ sacB+, gene replacement vector with MCS from pUC18 | Hoang et al. (1998) |
| pEX18GmGD | pEX18Gm with a SacI-BamHI 818-bp fragment from upstream operon goxAB and a 787-bp BamHI-PstI fragment from downstream the operon goxAB, both cloned in its MCS. | This study |
| pBSL182 | ori R6K, mob RP4, Apr; mini-Tn10 Gmr | Alexeyev and Shokolenko (1995) |
| pBlodA II | ori R6K, mob RP4, Apr; mini-Tn10 Kmr, lodA | Gomez (2010) |
| pBgoxAB | ori R6K, mob RP4, Apr; mini-Tn10 Kmr, goxAB | This study |
| Primers1 | ||
| GOXDIRSAC | 5′-ACGCTTTGGAGCTCATACTACTG-3′ | |
| GOXREVBAM | 5′-CGTCCTATCTGGATCCATTAATATGAAA-3′ | |
| GOXDIRBAM | 5′-CGCACAAGGATCCCTAACGGTTTC-3′ | |
| GOXREVPST | 5′-TATAGGGAGAACTGCAGGGGAAAAC-3′ | |
| GOSEC1 | 5′-GAAATCCCACCGGTTACAAC-3′ | |
| GOREVSEC2 | 5′-CCTCGGAGTTTGGACGTTG-3′ | |
| pGODIRKPN1 | 5′-TACACTTCAGGTACCTTCCTTATACAAC-3′ | |
| GOREVPST1 | 5′-CATTATCCGTTTTGACCTGCAGAGTGG-3′ | |
| GODIRNDE1 | 5′-GATAGGACGATCATATGCAAAATGACGG-3′ | |
| GOREVSMA1 | 5′-GTTTTGACCTACACCCGGGTTAATTGATG-3′ | |
1
Restriction sites are underlined.