Table 5. Candida glabrata strains and plasmids used in this study.
Strains/plasmids | Genotypes/descriptions | Sources |
Strains | ||
FMME019 | C. glabrata Δura3 Δarg8 mutant strain derived from C. glabrata CCTCC M202019 | [33] |
DA-0 | FMME019 pYES-PGK1 | This study |
DA-ILV2 | FMME019 pYES-PGK1-ILV2 | This study |
DA-1 | FMME019 pYES-PGK1-alsS | This study |
DA-2 | FMME019 Δilv5 pYES-PGK1-alsS | This study |
DA-3 | FMME019 Δilv5 Δbdh::arg8 pYES-PGK1-alsS | This study |
Plasmids | ||
pYES2 | 2μ, Amp, URA3, PGAL1 | Invitrogen |
pYES-PGK1 | 2μ, Amp, URA3, PPGK1 | This study |
pYES-PGK1-ILV2 | C. glabrata ILV2 gene under the control of PGK1 promoter in pYES-PGK1 | This study |
pYES-PGK1-alsS | Bacillus subtilis alsS gene under the control of PGK1 promoter in pYES-PGK1 | This study |