Figure 4. MALDI-TOF MS analysis of purified hGH from Trx-hGH in E. coli (no DTT during purification).
(A) Tryptic peptide map of hGH (191 aa). (B) MALDI-TOF MS for purified hGH in reducing conditions. (C) MALDI-TOF MS for the purified hGH in non-reducing conditions. MALDI-TOF MS analysis of reduced and non-reduced hGH obtained from MBP-hGH and PDIb′a′-hGH showed similar results (data not shown).
