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. 2014 Mar 10;9(3):e90544. doi: 10.1371/journal.pone.0090544

Figure 3. Hypoxia-mediated upregulation of mRNA expression of Cu transporters (CTR1, ATP7A) and lysyl oxidase (LOX) is associated with an increase in Cu transportation in human pulmonary arterial smooth muscle cells (PASMC).

Figure 3

A: Real-time RT-PCR analysis on ATP7A, CTR1, and LOX (left panel) and 64Cu uptake (mean±SE) in human PASMC exposed to normoxia (Nor) and hypoxia (Hyp, 3% O2 for 48 hrs, n = 3; right panel). B: Real-time RT-PCR analysis on ATP7A, CTR1, and LOX (left panel) and 64Cu uptake (mean±SE, right panel) in human PASMC treated with vehicle (Cont) and CoCl2 (100 µM for 48 hrs, n = 3; right pane). Lactate dehydrogenase (LDH) and erythropoietin (EPO) were used as positive controls. *P<0.05, **P<0.01, ***P<0.001 vs. Hyp or CoCl2.