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. 2014 Mar 10;9(3):e90544. doi: 10.1371/journal.pone.0090544

Figure 4. Downregulation of HIF-1α by siRNA significantly attenuates mRNA expression of CTR1 in hypoxic PASMC.

Figure 4

A: Real-time RT-PCR analysis on HIF-1α (a), HIF-2α (b) and CTR1 (c) in hypoxic PASMC treated with (50–200 pmol) or without (0 pmol) siRNA specifically targeting HIF-1α, HIF-2α and CTR1, respectively. Data are shown in mean±SE. ***P<0.01 vs. control hypoxic cells (0-pmol siRNA). B: Real-time RT-PCR analysis on human CTR1 (a), ATP7A (b), HIF-1α (c) and HIF-2α (d) in hypoxic PASMC treated with 100-pmol scrambled siRNA (Control-siRNA, open bars), HIF-1α-siRNA (solid bars), HIF-2α-siRNA (light grey bars), and CTR1-siRNA (dark grey bars), respectively. ***P<0.01 vs. hypoxic cells treated with scrambled siRNA (Control-siRNA).