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. 2014 Mar 10;9(3):e90544. doi: 10.1371/journal.pone.0090544

Figure 6. PASMC migration is dependent on Cu and inhibited by knockdown of CTR1.

Figure 6

Cell migration was determined by the modified Boyden chamber assay. Cells were plated on top of the porous (8-µm pore) membrane. After 48 hrs, the membrane was fixed and stained using Diff-Quick and the migrated cells in randomly chosen fields were counted at 200× magnification. A: Representative images (a) showing human PASMC cultured under normoxic (Nor) or hypoxic (Hyp) conditions in the absence (Control) or presence (BCS) of 200 µM BCS (a Cu chelator). Summarized data (mean±SE) showing migrated cell counts (b) in normoxic (Nor) and hypoxic (Hyp) PASMC treated with (BCS) or without (Control) BCS. **P<0.01 vs. Control. The Cu-dependent cell migration (c) was determined by the percent changes in migrated cell counts between Control and BCS-treated cells in Nor and Hyp. Data are shown as mean±SE. *P<0.05 vs. Nor. B: Representative images (a) showing human PASMC treated with scrambled siRNA (Control-siRNA) or CTR1-siRNA under Nor or Hyp conditions. Summarized data (mean±SE) showing migrated cell counts (b) in Nor and Hyp PASMC treated with Control-siRNA (solid bars) or CTR1-siRNA (open bars). **P<0.01 vs. Control. The Cu-dependent cell migration (c) was determined by the percent changes in migrated cell counts between PASMC treated with Control-siRNA and CTR1-siRNA in normoxia (Nor) and hypoxia (Hyp). Data are shown as mean±SE. *P<0.05 vs. Nor.