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. 2014 Mar 10;9(3):e90544. doi: 10.1371/journal.pone.0090544

Figure 8. Chelation of Cu diminishes human PASMC proliferation.

Figure 8

A: BrdU incorporation (mean±SE) in control PASMC and PASMC treated with aphidcolin (a DNA-replication inhibitor, 48 hrs) and 100 µM tetrathiomolybadate (TTM, a Cu chelator, 48 hrs). *P<0.05 vs. Control. Cells treated with aphidicolin were used as a negative control. B: Western blot analysis (left panel) on PCNA (a proliferating cell nuclear antigen) in control (Cont) PASMC and PASMC treated with the Cu chelators, BCS and TTM. Summarized data (right panel, mean±SE) showing PCNA protein levels in control cells, BCS-treated cells and TTM-treated cells. *P<0.05 vs. Control.