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. 2014 Mar 10;9(3):e90089. doi: 10.1371/journal.pone.0090089

Figure 4. Time-dependent expression of IL-6 and chemokines in the 3-NP-injured cochlea lateral wall.

Figure 4

Quantitative RT-PCR to examine the expression of IL-6 and chemokines, including CCL2 (MCP-1), CCL5 (RANTES) and CCL4 (MIP-1β), in the injured cochlear lateral wall. The expression of IL-6 was induced 3 h after 3-NP administration. IL-6 expression was higher in the PTS model (blue lines) than in the temporal threshold shift model (pink lines) at Day 1 (a; p<0.01; purple), suggesting that IL-6 was a detrimental factor. IL-6 expression was high in the PTS model at Day 1 post-injury but was quickly downregulated. Chemokines were induced in the 3-NP-injured lateral wall from 6 h to 7 d post 3-NP administration. Chemokine expression was induced more slowly than that of proinflammatory cytokines, but it was sustained (a–d). The level of CCL2 expression in the PTS and TTS models was significantly different, being greater in the PTS model than in the TTS model (b; p<0.05 (green) and p<0.01 (purple)), suggesting that CCL2 was also a detrimental factor. * indicates p<0.05, ** indicates p<0.01.