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. 2014 Mar 10;9(3):e90588. doi: 10.1371/journal.pone.0090588

Figure 3. Production of MSRB2-Bar and MSRB2-mini transformed rice.

Figure 3

(A) Schematic representation of the expression vectors that were used for rice transformation. CaMSRB2, pepper methionine sulfoxide reductase B2; Rab pro, rice responsive to ABA protein 21 promoter; PinIIter, potato proteinase inhibitor II terminator; 35S pro, cauliflower mosaic virus 35S promoter; Bar, phosphinothricin acetyltransferase; Noster, nopaline synthesis terminator. (B) RT-PCR analysis of CaMSRB2 gene expression in WT, MSRB2-Bar and MSRB2-mini plants under drought stress for 2 days. Rab21 and RbcS were used as a positive and negative control for drought stress, respectively, and tubulin was used as an internal control.