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. 2014 Mar 6;6:ecurrents.md.faafdde4bea8df4aa7d06cd5553119a6. [Version 1] doi: 10.1371/currents.md.faafdde4bea8df4aa7d06cd5553119a6

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geNorm analysis — Upper panel: Genes ranked by their calculated average expression stabilities (stability increases from left to right) in C2C12, 2B4 and SF1 (left to right, respectively). Lower panel: optimal number of control genes for normalization for C2C12, 2B4 and SF1 (left to right, respectively). Charts compare pairwise variation V of normalization factors as number of factors used increases (n/n+1). V2/3 thus compares 2 genes with 3, V3/4: 3 genes with 4. Values below 0.15 are assumed to be sufficient, thus in all cases increasing the number of genes used beyond 2 is of limited benefit.